9095-10111-115 ClearASeal Perf
ClearASeal Perf (115mm wide rolls)
Description
A clear, perforated gas permeable film, suited for cell and seed culture, with good optical clarity and moderate solvent resistance. The seal is non-peelable.
A clear, perforated gas permeable film, suited for cell and seed culture, with good optical clarity and moderate solvent resistance. The seal is non-peelable.
| PRODUCT CODE | SEAL TYPE | SIZE |
|---|---|---|
| 9095-10111-115LR | ClearASeal Perf | 500M x 115mm |
| 9095-10111-115SR | ClearASeal Perf - Sterile | 500M x 115mm |
| 9095-10111-115TR | ClearASeal Perf | 5M x 115mm |
Compatibility
Polypropylene (PP), Polyethylene (PE), Polystyrene (PS) and Cyclic Olefin Copolymer (COC) plates.
Application
Short-term incubation, agriculture and seed storage, insect storage, cell culture.
Storage
Store in a cool place. Avoid direct exposure to sunlight. It is recommended to use the seals within three years from date of purchase. Three years when stored at 21℃ (70℉), 50% relative humidity, out of direct sunlight, in original packaging.
Properties
Temperature range -80℃ to 80℃, or 110℃ with pressurised PCR heated lids.
Sealing
Temperature and Dwell Time: 180℃, 2 seconds
Recommended sealing equipment: Ultraseal Lite / PRO / XT PRO / ePRO, Wasp, Chameleon, Kube, ALPS3000/5000, A4S, PlateLoc.
SPECIFICATIONS
Visual Description
Temperature Range: -80℃ to +80℃ or 110℃ with pressurized PCR heated lids.
TEST PROCEDURES
Mass Loss
Confirming the materials ability to resist high temperatures. Results: Pass.
Details: Mass loss of solution evaluated after 30 cycles of 3 step PCR Programme. Equipment: ABI Thermocycler, Precision Balance.
Pierce
Measuring the force required to push a standardised needle through the material via compression measuring equipment. Results: N/A.
Details 5 tests run using a standardised needle, ensuring that less than 10N is required to pierce the surface & access the wells. Equipment Instron 3343 Tensometer.
Optical
Determining the materials optical clarity by measuring the transmission of emissive dye through the material. Results: Pass.
Details Record the light transmission of a sealed microplate using a Flurophore dye stock solution and a microplate reader. Equipment BMG Labtech - FluroStar.
Peel
Measuring the materials permanence of adhesion & its ability to be removed, via extension measuring equipment. Results: N/A.
Details Cohesive Failure, Adhesive Transfer, Material tear & Successful Peel are measured & recorded after a 180℃ peel test. Equipment Instron 3343 Tensometer.
Low Temperature Seal Test
Confirming the materials ability to resist low temperatures. Results: Pass.
Details: Microplates are sealed at specified low temperatures & subjected to a series of tests to substantiate seal integrity. Equipment: Laboratory Cold storage unit.
Solvent
Evaluating the materials resistance to solvents (DMSO used as an aggressive standard). Results: Pass.
Details: Sealed plate is subjected to a high concentration of DMSO for a time period at low temperatures after which seal damage & volume loss are determined. Equipment Laboratory Cold storage unit, DMSO solution.
Plate Types, Sealing Temp. Time Settings
Polypropylene (PP), Polyethylene (PE), Polystyrene (PS), Cyclo Olefin Copolymer (COC).
Details: Temperature and Dwell Time: 175℃, 2 seconds.
QC testing is carried out to ensure that products are free from nucleases (DNases & RNases) as well as human genomic DNA. Although every effort is made, including cleanroom manufacture, to maintain this level of cleanliness, best laboratory practice with regards to duplicate testing should be followed.
