9095-10103-115 ClearASeal Pierce
ClearASeal Pierce (115mm wide rolls)
Description
A clear heat-seal film which is ideal for use with ABI 3730 sequencer. The film has good optical clarity and moderate solvent resistance, it is non-peelable and pierceable.
A clear heat-seal film which is ideal for use with ABI 3730 sequencer. The film has good optical clarity and moderate solvent resistance, it is non-peelable and pierceable.
| PRODUCT CODE | SEAL TYPE | SIZE |
|---|---|---|
| 9095-10103-115LR | ClearASeal Pierce | 500M x 115mm |
| 9095-10103-115SR | ClearASeal Pierce - Sterile | 500M x 115mm |
| 9095-10103-115TR | ClearASeal Pierce | 5M x 115mm |
Compatibility
Polypropylene (PP), Polyethylene (PE), Polystyrene (PS) and Cyclic Olefin Copolymer (COC) plates.
Application
Recommended for use with the Abi 3730 Sequencer as the thinner structure pierces more easily.
Storage
Store in a cool place. Avoid direct exposure to sunlight. It is recommended to use the seals within three years from date of purchase. Three years when stored at 21℃ (70℉), 50% relative humidity, out of direct sunlight, in original packaging.
Properties
Temperature range -80℃ to 80℃ or 110℃ with pressurised PCR heated lids.
Sealing
Temperature and Dwell Time: 175℃, 2 seconds.
Recommended sealing equipment: Ultraseal Lite / PRO / XT PRO / ePRO, Wasp, Chameleon, Kube, ALPS3000/5000, A4S, PlateLoc.
SPECIFICATIONS
Visual Description
Clear and thick plastic seal. Sealing surface on inside of roll and is less reflective.
Physical Properties
Flexible plastic, difficult to crease, upper surface feels very smooth, sealing side has a slight rough feel. Temperature range -80℃ to 80℃ or 110℃ with pressurized PCR heated lids.
TEST PROCEDURES
Mass Loss
Confirming the materials ability to resist high temperatures. Results: Pass.
Details: Mass loss of solution evaluated after 30 cycles of 3 step PCR Programme. Equipment: ABI Thermocycler, Precision Balance.
Pierce
Measuring the force required to push a standardised needle through the material via compression measuring equipment. Results: Pass.
Details 5 tests run using a standardised needle, ensuring that less than 10N is required to pierce the surface & access the wells. Equipment Instron 3343 Tensometer.
Optical
Determining the materials optical clarity by measuring the transmission of emissive dye through the material. Results: Pass.
Details Record the light transmission of a sealed microplate using a Flurophore dye stock solution and a microplate reader. Equipment BMG Labtech - FluroStar.
Peel
Measuring the materials permanence of adhesion & its ability to be removed, via extension measuring equipment. Results: N/A.
Details Cohesive Failure, Adhesive Transfer, Material tear & Successful Peel are measured & recorded after a 180℃ peel test. Equipment Instron 3343 Tensometer.
Low Temperature Seal Test
Confirming the materials ability to resist low temperatures. Results: Pass.
Details: Microplates are sealed at specified low temperatures & subjected to a series of tests to substantiate seal integrity. Equipment: Laboratory Cold storage unit.
Solvent
Evaluating the materials resistance to solvents (DMSO used as an aggressive standard) Results: N/A Details Sealed plate is subjected to a high concentration of DMSO for a time period at low temperatures after which seal damage & volume loss are determined. Equipment Laboratory Cold storage unit, DMSO solution.
Plate Types, Sealing Temp. Time Settings
Polypropylene(PP), Polyethylene (PE), Polystyrene (PS), Cyclo Olefin Copolymer (COC) Details: Temperature and Dwell Time: 175℃, 2 seconds.
QC testing is carried out to ensure that products are free from nucleases (DNases & RNases) as well as human genomic DNA. Although every effort is made, including cleanroom manufacture, to maintain this level of cleanliness, best laboratory practice with regards to duplicate testing should be followed.
