A "stick to all" peel-able, foil laminate heat-seal film which is suited for all plate types - Polypropylene (PP), Polystyrene (PS) and Cyclo Olefin Copolymer (COC). The film has a good liquid barrier and high resistance to solvents. It is peel-able (from -80˚C freezer) and is non-pierceable. This seal has a white colour to the top aspect.

Compatibility
Polypropylene (PP), Polystyrene (PS) & Cyclo Olefin Copolymer (COC) plates.

Application
PCR, low temperature, short term compound storage, short term room temperature compound storage (less than 5 days).

Storage
Store in a cool place. Avoid direct exposure to sunlight. It is recommended to use the seals within three years from date of purchase. Three years when stored at 21˚C (70˚F), 50% relative humidity, out of direct sunlight, in original packaging.

Properties
Temperature range -80˚C to 110˚C.

Sealing
Temperature and Dwell Time: 175˚C, 2 seconds Recommended sealing
Equipment: * Efly, Kseal, 4s2 ** Wasp, ThermoALPS300/3000, Kube, Flexiseal,
Chameleon, REMP (PHS) *** Agilent VII Plateloc, REMP (LHS/SHS).

SPECIFICATIONS

Visual Description
Metallic with upper surface gloss white. Seal surface metallic burnished foil.

Physical Properties
Flexible, not easily creased. Thicker than IST-104. Temperature Range: -80˚C to +110˚C.

TEST PROCEDURES

Mass Loss
Confirming the materials ability to resist high temperatures. Results: Pass.
Details: Mass loss of solution evaluated after 30 cycles of 3 step PCR Programme. Equipment: ABI Thermocycler, Precision Balance.

Pierce
Measuring the force required to push a standardised needle through the material via compression measuring equipment. Results: N/A.
Details 5 tests run using a standardised needle, ensuring that less than 10N is required to pierce the surface & access the wells. Equipment Instron 3343 Tensometer.

Optical
Determining the materials optical clarity by measuring the transmission of emissive dye through the material. Results N/A.
Details Record the light transmission of a sealed microplate using a Flurophore dye stock solution and a microplate reader. Equipment BMG Labtech - FluroStar.

Peel
Measuring the materials permanence of adhesion & its ability to be removed, via extension measuring equipment. Results: Pass.
Details Cohesive Failure, Adhesive Transfer, Material tear & Successful Peel are measured & recorded after a 180˚ peel test. Equipment Instron 3343 Tensometer.

Low Temperature Seal Test
Confirming the materials ability to breath. Results: Pass.
Details: Microplates are sealed at specified low temperatures & subjected to a series of tests to substantiate seal integrity. Equipment: Laboratory Cold storage unit.

Solvent
Evaluating the materials resistance to solvents (DMSO used as an aggressive standard) Results: Pass.
Details Sealed plate is subjected to a high concentration of DMSO for a time period at low temperatures after which seal damage & volume loss are determined. Equipment Laboratory Cold storage unit, DMSO solution.

Plate Types, Sealing Temp. Time Settings
Polypropylene (PP), Polyethylene (PE), Polystyrene (PS), Cyclo Olefin Copolymer (COC) and non-binding coated plates.
Temperature and Dwell Time: 175˚C, 2 seconds.

QC testing is carried out to ensure that products are free from nucleases (DNases & RNases) as well as human genomic DNA. Although every effort is made, including cleanroom manufacture, to maintain this level of cleanliness, best laboratory practice with regards to duplicate testing should be followed.